One hundred ten labs around the world can run this assay, and can tell if an isolate contains polio, or not, and if so, what kind.Ĭlick here for hi-resolution image (14. Bioz Stars score: 86/100, based on 1 PubMed citations. Spectral data were captured and analyzed by using Rotor-Gene Real-Time Analysis Software 6.0 Build 14. Specific probes bind to the DNA, in order to determine what type of polio present. Corbett Rotor Gene 6000 Series Software Version 1 7, supplied by Qiagen, used in various techniques. Corbett Real-Time PCR Machine with the Rotor-Gene 6000 Series Software 1.7 (QIAGEN, the Netherlands) using SYBR Green method. QIAGEN will no longer support Rotor-Gene 3000 instruments and Rotor-Gene 6. Rotor-Gene 6000 software requires a computer with Windows XP or Vista operating system. Status: AVAILABLE Last checked: 16 Minutes ago In order to read or download corbett rotor-gene 6000 manual version 1. In the instrument, viral RNA is copied into DNA and then the DNA is amplified. The Rotor-Gene Q software v2.3 or higher versions can run with Windows XP or Windows 7 32-bit and 64-bit operating systems. File Name: corbett rotor-gene 6000 manual version 1.7.pdf Size: 2029 KB Type: PDF, ePub, eBook Category: Book Uploaded:, 19:46 PM Rating: 4.6/5 from 730 votes. It is not necessary to use expensive kits that are specific to the instrument. Most DNA amplification enzymes/ buffers can be used on the system to generate Quantitation/Melt data. The amplified product is purified for bidirectional. The themocycler can vary the temperature, which is important, for PCR requires multiple test rounds at different temperatures. The Rotor-Gene multi-filter system can detect all available real-time chemistries including Sybr-Green, dual-labelled and MGB probes, FRET and Molecular Beacons. If the mycelia do not pellet, the material is contained with a. The data from this analysis is stored in a computer, while the software further analyzes the data before being reviewed by a scientist. This image depicted a Centers for Disease Control and Prevention (CDC) scientist placing samples into a real-time PCR machine, known as a Corbett Research Rotor-Gene 6000 thermocycler, in order to identify the various types of poliovirus contained therein.
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